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Plant & Animal Whole Genome Resequencing

Whole-genome re-sequencing products for animals and plants leverage advanced sequencing technologies to facilitate precise genetic mapping, aiding in species improvement, trait selection, and biodiversity conservation

Work flow

workflow-image

Technical Parameters

Sequencing rangeWhole genome region
Sequencing strategyNGS PE150
Sequencing throughput10x-30x
Data qualityFastq files, Q30≥85%
Data analysisStandard+advanced analysis
TATStandard: 25 WD

Applications

Genetic Diversity and Population Genetics ResearchBreeding Improved VarietiesEcological and Environmental Adaptability Research
Disease-Related Gene IdentificationFunctional Genomics ResearchConservation Genetics

Bioinformatics Analysis

1.Process the original data to remove adapters, contaminating sequences and low-quality reads.
2.Data alignment using the BWA software and a reference genome.
3.Statistical analysis of data production, analysis of sequencing depth, and uniformity of coverage.
4.Detection of SNP variations using GATK.
5.Annotation of SNPs with RefGene.
6.Statistical analysis of the distribution of SNPs across various gene functional elements.
7.Detection of InDel variations using GATK.
8.Annotation of InDels with RefGene.
9.Statistical analysis of the distribution of InDels across various gene functional elements.
10.Detection and annotation of Structural Variations (SVs).
11.Detection and annotation of Copy Number Variations (CNVs).

Advantages

yesMultiple sample types(FFPE, cfDNA, ctDNA)
yesPCR/PCR free library construction provided
yes100ng minimum DNA input
yesAdvanced analysis & customized analysis provided

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