Product Overview

Hydrophilic Untargeted Metabolomics is designed for the comprehensive analysis of hydrophilic (highly polar) small-molecule metabolites, such as amino acids, organic acids, and TCA cycle intermediates.
By utilizing advanced instrumentation, including more hydrophilic columns, and optimized bioinformatics workflows, it significantly enhances the identification and coverage of these challenging-to-analyze compounds. This enables deeper insights into metabolic pathways and facilitates the discovery of relevant biomarkers.

Advantages

Advanced MS platforms: Thermo Scientific Q Exactive, Q Exactive Plus, Orbitrap Exploris 120/240, AB SCIEX TripleTOF 6600 for high sensitivity and accuracy.
Well-Curated Large Database: Integrated public (HMDB, MassBank, GNPS), commercial, and in-house databases for reliable identification.
Rigorous QC: Blank controls, m/z/retention time stability monitoring, signal intensity validation, PCA analysis, and correlation coefficient assessment.
Stringent Laboratory Management: Sample traceability, ice-cold processing, and environmental control for consistency.
High Identification Efficiency: Average of 1,000+ metabolites identified per blood sample, with small-molecule metabolites accounting for ~80%.
Innovative Workflow: Combines hydrophilic columns with advanced bioinformatics analysis for faster, more accurate results.

Product Parameters

Sample Requirements

workflow

Analysis Contents

Application Scenarios

Biomarker Discovery for Metabolic Diseases
Pharmacological and Toxicological Mechanism Research
Nutrigenomics and Gut Microbiota Studies
Cancer Metabolomics Research
Plant and Microbial Metabolomics

FAQ

Q：What compounds does it focus on?

A： It specializes in highly polar metabolites, including amino acids, organic acids, TCA cycle intermediates, and other hydrophilic small molecules.

Q：Which sample types are applicable?

A： Compatible with common samples like blood, urine, tissue, and cell extracts. Custom protocols can be designed for specific sample matrices.

Q：How is data accuracy guaranteed?

A： Through optimized workflows (hydrophilic columns, tailored bioinformatics) and integration of databases (HMDB, MassBank, in-house), plus strict QC for m/z, retention time, and signal intensity.

Q：What’s the typical identification throughput?

A： For blood samples, an average of 1,000+ metabolites are identified, with small-molecule metabolites accounting for ~80%.

Q：What should I do if I focus on specific target compounds?

A：
1.First, confirm with technical support whether targeted metabolic detection is feasible for your target compounds.
2.Note that untargeted metabolomics only provides relative quantification (not absolute quantification) and has limitations in qualitative accuracy.
3.Ask technical support to verify if your concerned substances are included in the curated database and if they can be detected in your specific sample type.