Product Overview

The 10X single-cell technology, based on microfluidics and droplet technology, uses an oil-in-water system to encapsulate cells with barcoded gel beads into Gel Beads in Emulsion (GEMs) for separation and labeling. It constructs high-throughput sequencing libraries and is widely used in studying cell heterogeneity, analyzing tumor microenvi-ronments, and detecting immune cell populations.

Advantages

(1) Experience in dissociating tissue samples of over 200 tumor types
(2) Multi-omics joint analysis solution
(3) Over 10 unique bioinformatics algorithms
(4) Clinical-grade experimental management

Product Parameters

Sample Requirements

Workflow

Analysis Contents

Application Scenarios

Tumor Microenvironment Research
Developmental Biology
Neuroscience
Immunology and Infection
Disease Mechanism Research
Drug Discovery and Toxicology
Stem Cell and Regenerative Medicine

FAQ

Q: Why is the requirement for cell viability so high after the preparation of single-cell suspension?

A： RNA from dead cells will be released into the extracellular fluid or mixed into the GEMs of living cells. These free RNAs may be encapsulated together with the cells, affecting subsequent reactions and ultimately leading to inaccurate analysis results. Meanwhile, a high number of dead cells will also lead to inaccurate estimation of cell quantity and affect the capture rate.

Q: How many cells can be sequenced in one run of 3' single-cell transcriptome sequencing? What is the capture efficiency of a single cell? Is there a possibility of capturing multiple cells?

A： Different platforms are equipped with microfluidic chips with 1–8 channels. Taking 10X as an example, a single chip can sequence up to 8 samples simultaneously. Each channel can process 100–10,000 cells, so a single chip can obtain 100–80,000 cells at one time.Under general experimental conditions, the single-cell capture efficiency of 3' single-cell transcriptome sequencing is as high as 65%. The probability of capturing multiple cells is extremely low, about 0.9% per 1000 cells.

Q: What results can be obtained from the standard analysis of scRNA sequencing?

A： The standard analysis pipeline includes quality control of sequencing data and statistics of various indicators (such as cell number, number of detected genes, data volume per single cell, etc.), gene expression quantification, cell type annotation, screening of differentially expressed genes and their functional annotation. In addition, personalized analysis can be performed based on the project background and experimental design.

Q: What common tissue types require nuclear extraction for single-nucleus transcriptome sequencing?

A： Brain tissues containing neurons, liver tissues targeting hepatocytes, lung tissues focusing on lung epithelial cells, adipose tissues for adipocytes, heart tissues for cardiomyocytes, muscle tissues for muscle cells, spinal cord/brain tissues targeting neurons, and kidney tissues focusing on podocytes, etc. At present, plant samples also adopt nuclear extraction for single-cell transcriptome research, which reduces the impact of stress gene expression and the interference of cytoplasmic metabolites, and is compatible with cells of larger diameters.

Q: Can fresh tissues be frozen after being placed in preservation solution for single-nucleus transcriptome sequencing?

A： No. This operation will form ice crystals and damage cells and cell nuclei.