Untargeted Metabolomics Mix is a balanced solution for global profiling of small-molecule metabolites (<1500 Da), focusing on moderately polar compounds. It enables efficient identification and quantification of common metabolites and lipids via LC-HRMS and high-throughput techniques.
This product systematically analyzes large metabolomic datasets to reveal profile variations, correlate with phenotypes, and integrate advanced analyses (e.g., pathway enrichment, diagnostic models). It supports biomarker screening, pharmacological/toxicological evaluation, and mechanism research, delivering comprehensive insights into biological systems.

Advantages

Advanced MS platforms: Thermo Scientific Q Exactive, Q Exactive Plus, Orbitrap Exploris 120/240, AB SCIEX TripleTOF 6600.
Well-Curated Large Database: Integrated public databases (HMDB, MassBank, GNPS), Commercial databases and in-house database.
Metabolite identification: Average >1,000 metabolites in blood and >1,500 in fecal samples.
Rigorous QC: Blank controls, m/z/retention time stability monitoring, signal intensity validation, PCA analysis, and correlation coefficient assessment.
Large-scale expertise: Proven capability in 15,000- and 22,000-sample cohort projects with real-time QC and advanced bioinformatics.

Product Parameters

Indicator	Parameter
Instrument Platform	Thermo Scientific Q Exactive, Q Exactive Plus, Orbitrap Exploris 120/240, AB SCIEX TripleTOF 6600
Turnaround Time	20 Working Days
Delivery Standard	Raw Data + Standard Analysis

Sample Requirements

Sample Type	Recommended Sample Volume	Minimum Acceptable Sample Volume
Serum/Plasma/Cerebrospinal Fluid (CSF)/ Amniotic Fluid/Semen	200 µL	20 µL
Urine/Bile/Tears/Aqueous Humor/Saliva	1 mL	20 µL
Clinical and Animal Tissue Samples	100 mg	20 mg
Hard Tissues (e.g., Cartilage)	200 mg	20 mg
Cells	5×10⁶	5×10⁶
Exosomes	1×10⁷	1×10⁶
Feces/Intestinal Contents	100 mg	20 mg
Soil/Dry Wood Chips	2g	20 mg
Plant Leaves/Fruits/Pollen/Seeds	100 mg	20 mg
Plant Roots/Stems/Barks and Mushroom Fungi (e.g., Mushrooms,Auricularia Auricula)	500 mg	20 mg
Cell Supernatant/Microbial Culture Medium	500 µL	20 µL
Yeast/Mold Fungi,Non-Pathogenic Bacteria/Algal Microorganisms	100 mg	20 mg

workflow

Analysis Contents

Application Scenarios

Biomarker discovery
Drug pharmacology, toxicology, and metabolism
Food and agricultural research
Nutrition and metabolic diseases
Environmental toxicology and ecology
Botanical and traditional medicine
Microbiology and microbiome

FAQ

Q：What is the Untargeted Metabolomics Mix?

A： A balanced LC-MS-based solution for comprehensive profiling of small-molecule metabolites (<1500 Da), with a focus on moderately polar compounds. It enables efficient identification and quantification of common metabolites and lipids.

Q：What can this product help researchers achieve?

A： It helps reveal metabolic profile variations across samples, correlate them with phenotypic information, and supports advanced analyses like pathway enrichment and biomarker screening.

Q：What are its typical applications?

A： It is used for mechanism research, disease biomarker discovery, and pharmacological or toxicological evaluation.

Q：What should I do if I focus on specific target compounds?

A：
1.First, confirm with technical support whether targeted metabolic detection is feasible for your target compounds.
2.Note that untargeted metabolomics only provides relative quantification (not absolute quantification) and has limitations in qualitative accuracy.
3.Ask technical support to verify if your concerned substances are included in the curated database and if they can be detected in your specific sample type.